Ensemble studies have contributed tremendously to comprehending biological reactions. However, these studies characterize the average molecular population, and have limited ability in detecting intermediate states or distinguishing heterogeneities. Over the past few decades, single-molecule techniques, including fluorescence resonance energy transfer (FRET), magnetic tweezers (MT) and optical tweezers (OT), have proven to be exceedingly powerful in addressing this knowledge gap. By studying one molecule at a time, these approaches have enabled significant advancement in the understanding of a wide variety of biomolecular systems, especially those involving DNA and associated proteins. Our research interests are focused on understanding the mechanisms of molecular motors in the process of DNA replication, repair and transcription, such as helicase, polymerase and nuclease, by using these single‐molecule techniques and developing technological innovations to meet the challenges in the pursuits. In addition, we are working on understanding structures and functions of organelles and RNA-binding proteins. Our lab currently covers four major research fields.